About this Medicine article

Extracellular Mg2+- and Ca2+-sensing in mouse distal convoluted tubule cells by Bapty, Brian W.; Dai, Long-jun; Ritchie, Gordon; Jirik, Frank; Canaff, Lucie; Hendy, Geoffrey N.; Quamme, Gary A. is a Medicine article available to read on EtoBox.

An immortalized cell line (designated MDCT) has been extensively used to investigate the cellular mechanisms of electrolyte transport within the mouse distal convoluted tubule. Mouse distal convoluted tubule cells possess many of the functional characteristics of the in vivo distal convoluted tubule. In the present study, we show that MDCT cells also possess a polyvalent cation-sensing mechanism that is responsive to extracellular magnesium and calcium. Southern hybridization of reverse transcribed-polymerase chain reaction (RT-PCR) products, sequence determination and Western analysis indicated that the calcium-sensing receptor (Casr) is expressed in MDCT cells. Using microfluorescence of single MDCT cells to determine cytosolic Ca2+ signaling, it was shown that the polyvalent cation-sensing mechanism is sensitive to extracellular magnesium concentration ([Mg2+]o) and extracellular calcium concentration ([Ca2+]o) in concentration ranges normally observed in the plasma. Moreover, both [Mg2+]o and [Ca2+]o were effective in generating intracellular Ca2+ transients in the presence of large concentrations of [Ca2+]o and [Mg2+]o, respectively. These responses are unlike those observed f

Author

Bapty, Brian W.; Dai, Long-jun; Ritchie, Gordon; Jirik, Frank; Canaff, Lucie; Hendy, Geoffrey N.; Quamme, Gary A.

Publisher

Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; ScienceOpen (ISSN 0085-2538)

Published

1998

Language

EN

Field

Medicine (Health Sciences)