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This study investigates the use of cationic polyethylenimine (PEI) for gene transfection in the protozoan parasite Toxoplasma gondii, utilizing PEI/DNA polyplexes to deliver green fluorescent protein (GFP). The results demonstrate that varying the N/P ratio of the polyplexes influences their size, surface charge, and transfection efficiency, with optimal GFP expression observed at an N/P ratio of 30. The findings suggest that PEI is an effective and cost-efficient method for gene delivery in T. gondii, whic
- Author
- pagahev732
- Language
- EN