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CTGF/Hcs24, hypertrophic chondrocyte‐specific gene product, interacts with perlecan in regulating the proliferation and differentiation of chondrocytes by Takashi Nishida; Satoshi Kubota; Tomohiro Fukunaga; Seiji Kondo; Gen Yosimichi; Tohru Nakanishi; Teruko Takano‐Yamamoto; Masaharu Takigawa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Connective tissue growth factor/hypertrophic chondrocyte‐specific gene product 24 (CTGF/Hcs24) plays important roles in the control of the proliferation and differentiation of chondrocytes in vitro. To clarify the mechanisms of regulation by CTGF/Hcs24 with respect to cartilage metabolism, we investigated the interaction between CTGF/Hcs24 and heparan sulfate proteoglycan perlecan. An immunofluorescence study showed that CTGF/Hcs24 was colocalized with heparan sulfate and perlecan in human chondrosarcoma‐derived chondrocytic cell line HCS‐2/8 in vitro. Northern blot analysis showed that perlecan, syndecan‐1, ‐2, and ‐4 transcripts were detected in HCS‐2/8 cells. Particularly, expression of the perlecan gene increased markedly in HCS‐2/8 cells by recombinant CTGF/Hcs24 (rCTGF/Hcs24) treatment. We also found that CTGF/Hcs24 interacted with perlecan from HCS‐2/8 cells in vitro. Furthermore, CTGF/Hcs24‐stimulated gene expression of the aggrecan gene, as well as DNA/proteoglycan synthesis, was diminished when HCS‐2/8 cells were pretreated with heparinase, indicating that the effects of CTGF/Hcs24 on chondrocytes occurred through the interaction between CTGF/Hcs24 and heparan
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- Author
- Takashi Nishida; Satoshi Kubota; Tomohiro Fukunaga; Seiji Kondo; Gen Yosimichi; Tohru Nakanishi; Teruko Takano‐Yamamoto; Masaharu Takigawa
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)