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Proton translocation coupled to formate oxidation in anaerobically grown fermenting Escherichia coli by Marta Hakobyan; Harutjun Sargsyan; Karine Bagramyan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Proton translocation, coupled to formate oxidation and hydrogen evolution, was studied in anaerobically grown fermenting Escherichia coli JW136 carrying hydrogenase 1 (hya) and hydrogenase 2 (hyb) double deletions. Rapid acidification of the medium by EDTA-treated anaerobic suspension of the whole cells or its alkalization by inverted membranes was observed in response to application of formate. The formate-dependent proton translocation and 2H(+)-K(+) exchange coupled to H(2) evolution were sensitive to the uncoupler, carbonylcyanide-m-chlorophenylhydrazone, and to copper ions, inhibitors of hydrogenases. No pH changes were observed in a suspension of formate-pulsed aerobically grown ("respiring") cells. The apparent H(+)/formate ratio of 1.3 was obtained in cells oxidizing formate. The 2H(+)-K(+) exchange of the ATP synthase inhibitor N,N'-dicyclohexylcarbodiimide-sensitive ion fluxes does take place in JW136 cell suspension. Hydrogen formation from formate by cell suspensions of E. coli JW136 resulted in the formation of a membrane potential (Deltapsi) across the cytoplasmic membrane of -130 mV (inside negative). This was abolished in the presence of copper ions, although they h

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Author

Marta Hakobyan; Harutjun Sargsyan; Karine Bagramyan

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)