Can I read Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells on EtoBox?
Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells by Elizabeth M. Muir; Ian Fyfe; Sonya Gardiner; Li Li; Philippa Warren; James W. Fawcett; Roger J. Keynes; John H. Rogers is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells about?
Although many eukaryotic proteins have been secreted by transfected bacterial cells, little is known about how a bacterial protein is treated as it passes through the secretory pathway when expressed in a eukaryotic cell. The eukaryotic N-glycosylation system could interfere with folding and secretion of prokaryotic proteins whose sequence has not been adapted for glycosylation in structurally appropriate locations. Here we show that such interference does indeed occur for chondroitinase ABC from the bacterium Proteus vulgaris, and can be overcome by eliminating potential N-glycosylation sites. Chondroitinase ABC was heavily glycosylated when expressed in mammalian cells or in a mammalian translation system, and this process prevented secretion of functional enzyme. Directed mutagenesis of selected N-glycosylation sites allowed efficient secretion of active chondroitinase. As these proteoglycans are known to inhibit regeneration of axons in the mammalian central nervous system, the modified chondroitinase gene is a potential tool for gene therapy to promote neural regeneration, ultimately in human spinal cord injury.
Who reads Modification of N-glycosylation sites allows secretion of bacterial chondroitinase ABC from mammalian cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Elizabeth M. Muir; Ian Fyfe; Sonya Gardiner; Li Li; Philippa Warren; James W. Fawcett; Roger J. Keynes; John H. Rogers
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0168-1656)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)