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Rapid Stable Isotope Dilution Analysis of Very-long-chain Fatty Acids, Pristanic Acid and Phytanic Acid Using Gas Chromatography–electron Impact Mass Spectrometry by P Vreken; A.E.M van Lint; A.H Bootsma; H Overmars; R.J.A Wanders; A.H van Gennip is a Chemistry article available to read on EtoBox.

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A common feature of most peroxisomal disorders is the accumulation of very-long-chain fatty acids (VLCFAs) and / or pristanic and phytanic acid in plasma. Previously described methods utilizing either gas chromatography alone or gas chromatography-mass spectrometry are, in general, time-consuming and unable to analyze VLCFAs, pristanic and phytanic acid within a single analysis. We describe a simple, reproducible and rapid method using gas chromatography / mass spectrometry with deuterated internal standards. The method was evaluated by analysing 30 control samples and samples from 35 patients with defined peroxisomal disorders and showed good discrimination between controls and patients. This method is suitable for routine screening for peroxisomal disorders.

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Author

P Vreken; A.E.M van Lint; A.H Bootsma; H Overmars; R.J.A Wanders; A.H van Gennip

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)