Can I read Sensitive Acid Phosphatase Assay Based on Light-activated Specific Oxidase Mimic Activity on EtoBox?

Sensitive Acid Phosphatase Assay Based on Light-activated Specific Oxidase Mimic Activity by Xiaohong Qiao; Hanmei Li; Huijun Ma; Han Zhang; Lihua Jin is a Chemistry article available to read on EtoBox.

What is Sensitive Acid Phosphatase Assay Based on Light-activated Specific Oxidase Mimic Activity about?

Acid phosphatase (ACP) is a key marker in the diagnosis of many diseases. However, exploiting a simple and sensitive sensor for the real-time quantitative analysis of ACP is still challenging. Herein, we attempted to develop a sensitive colorimetric sensing strategy for the detection of ACP based on light-activated oxidase mimic property of carbon dots (CDs). The synthesized CDs were proved to be capable of intrinsic light-activated oxidase mimic activity, which could generate reactive oxygen species to oxidize chromogenic substrate under ultraviolet light stimulation. Interestingly, this light-activated oxidase mimic behavior would be effectively suppressed by the antioxidant ascorbic acid (AA), a product from the hydrolysis of 2-phospho-L-ascorbic acid trisodium (AAP) mediated by ACP. Based on the above property, a facile and sensitive colorimetric sensing method for ACP was developed. Under the optimal conditions, the linear range for ACP 0.1–5.5 U/L, and the detection limit was 0.056 U/L. Compared with conventional nanozyme based ACP assay systems, the catalytic activity of light-activated nanozyme could be conveniently regulated by switching the light on and off, which made it

Who reads Sensitive Acid Phosphatase Assay Based on Light-activated Specific Oxidase Mimic Activity?

Author

Xiaohong Qiao; Hanmei Li; Huijun Ma; Han Zhang; Lihua Jin

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Chemistry (Physical Sciences)