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Can I read Further analysis of the involvement of the envelope anion channel PIRAC in chloroplast protein import on EtoBox?
Further analysis of the involvement of the envelope anion channel PIRAC in chloroplast protein import by Paul W. J. van den Wijngaard; Jeroen A. A. Demmers; Simon J. Thompson; Hans L. J. Wienk; Ben de Kruijff; Wim J. Vredenberg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Further analysis of the involvement of the envelope anion channel PIRAC in chloroplast protein import about?
The ability of preferredoxin to inactivate a 50-pS anion channel of the chloroplast inner membrane in the presence of an energy source was investigated using single-channel recordings. It was found that preferredoxin cannot inactivate the channel when GTP is the only energy source present. From this it is concluded that the precursor has to interact with the, translocon of the inner membrane of chloroplasts (Tic) complex to be able to inactivate the 50-pS anion channel. The ability of two mutants of preferredoxin with deletions in their transit sequence to inactivate the channel was also tested. Both mutants have been shown to have a similar binding affinity for the chloroplast envelope, but only one is able to fully translocate. The mutants were both able to inactivate the channel in a similar manner. From this it is concluded that full translocation is not necessary for the inactivation of the channel. It is also shown that preferredoxin is capable of inactivating the 50-pS anion channel in the chloroplast-attached configuration as was previously found in the inside-out configuration. From this it is concluded that stromal factors do not influence the protein-import-induced inact
Who reads Further analysis of the involvement of the envelope anion channel PIRAC in chloroplast protein import?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Paul W. J. van den Wijngaard; Jeroen A. A. Demmers; Simon J. Thompson; Hans L. J. Wienk; Ben de Kruijff; Wim J. Vredenberg
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)