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Leptospira Spp. Possess a Complete Haem Biosynthetic Pathway and Are Able to Use Exogenous Haem Sources by Rozenn Guégan; Jean-Michel Camadro; Isabelle Saint Girons; Mathieu Picardeau is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Summary Unlike the spirochetes __Borrelia burgdorferi__ and __Treponema pallidum__, __Leptospira__ spp. contain genes encoding the enzymes for most biosynthetic pathways. In this study, we describe the first haem biosynthetic pathway genes in the order __Spirochaetales__. Sequence analysis of the __L. interrogans__ genome shows that all haem biosynthetic genes (__hemA__, __heml__, __hemB__, __hemC__, __hemE__, __hemN__, __hemY__ and __hemH__) are clustered in a 15 kb region of the CII secondary chromosome. Although no __hemD__ homologue (encoding uroporphyrinogen III synthase) was found in the genome, the __L. interrogans hemC__ gene (encoding porphobilinogen deaminase) was able to restore uroporphyrinogen III synthase activity in an __Escherichia coli__Δ__hemD__ mutant, suggesting that the __L. interrogans hemC__ gene encodes a bifunctional enzyme. Similarly, we show that the __L. interrogans hemH__ gene (encoding ferrochelatase, the terminal enzyme of the haem biosynthetic pathway) is able to complement a ferrochelatase‐defective __E. coli__Δ__hemH__ mutant. Further investigation of ferrochelatases was undertaken in both saprophytic and pathogenic species of __Leptospira__. Fe

Who reads Leptospira Spp. Possess a Complete Haem Biosynthetic Pathway and Are Able to Use Exogenous Haem Sources?

Author

Rozenn Guégan; Jean-Michel Camadro; Isabelle Saint Girons; Mathieu Picardeau

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)