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Danshen injection induces autophagy in podocytes to alleviate nephrotic syndrome via the PI3K/AKT/mTOR pathway by Junqi Chen; Shengliang Yuan; Jie Zhou; Xiuye Huang; Wenjia Wu; Yiwen Cao; Hong Liu; Qinghong Hu; Xiaojie Li; Xueping Guan; Simin Yin; Jiaying Jiang; Yuan Zhou; Jiuyao Zhou is a Medicine article available to read on EtoBox.

What is Danshen injection induces autophagy in podocytes to alleviate nephrotic syndrome via the PI3K/AKT/mTOR pathway about?

## Background: Danshen injection (dsi) is an agent extracted from the salvia miltiorrhiza bunge, a natural drug commonly used to alleviate kidney diseases. however, the material basis and therapeutic effects of dsi on nephrotic syndrome (ns) remain unclear. ## Purpose: To investigate the material basis of dsi and the therapeutic effects and underlying mechanisms of ns. ## Methods: Ns models were established using adriamycin-induced balb/c mice and lipopolysaccharide-induced mouse podocytes (mpc-5). following dsi and prednisone administration, kidney coefficients, 24 h urine protein, blood urea nitrogen, and serum creatinine levels were tested. histomorphology was observed by periodic acid-schiff staining and hematoxylin and eosin staining of the kidney sections. the glomerular basement membrane and autophagosomes of the kidneys were observed using transmission electron microscopy. nephrin and desmin levels in the glomeruli were tested using immunohistochemistry. the viability of mpc-5 cells was tested using cell counting kit-8 after chloroquine and rapamycin administration in combination with dsi. the in vivo and in vitro protein levels of phosphatidylinositol 3-kinase (pi3k), akt,

Who reads Danshen injection induces autophagy in podocytes to alleviate nephrotic syndrome via the PI3K/AKT/mTOR pathway?

Author

Junqi Chen; Shengliang Yuan; Jie Zhou; Xiuye Huang; Wenjia Wu; Yiwen Cao; Hong Liu; Qinghong Hu; Xiaojie Li; Xueping Guan; Simin Yin; Jiaying Jiang; Yuan Zhou; Jiuyao Zhou

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Medicine (Life Sciences)