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Seeding Cells in Seahorse XFp Plates by javier.front is a document available to read on EtoBox.
1. Seeding adherent cells for metabolic assays requires using Seahorse XFp Miniplates, which have smaller well surfaces than standard plates. Miniplates should be seeded in carrier trays and have sterile water added to the surrounding moat. 2. Cells are seeded at concentrations optimized for the individual cell line, typically between 5,000-40,000 cells per well. Background wells are seeded with media only. 3. Cells are harvested, counted, diluted to the desired concentration, and 80uL is added to wells
- Author
- javier.front
- Language
- EN