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Highly sensitive detection of multiple proteins from single cells by MoS2-FET biosensors by Junqing Wei; Zhihan Zhao; Kuibo Lan; Zhi Wang; Guoxuan Qin; Ruibing Chen is a Chemistry article available to read on EtoBox.

What is Highly sensitive detection of multiple proteins from single cells by MoS2-FET biosensors about?

Single-cell analysis of proteins is critical to gain precise information regarding the mechanisms that dictate the heterogeneity in cellular phenotypes and their differential response to internal and external stimuli. However, tools that allow sensitive and easy measurement of proteins in individual cells are still limited. The emerging semiconductor-based bioelectronics may provide a new approach to overcome the challenges in this field, however its utility in single-cell protein analysis has not been explored. In this study, we investigated multiple protein detection in single cells by MoS 2 field effect transistors (MoS 2 -FETs) modified with specific biological probes. First, β-actin antibody was connected to the surface of MoS 2 -FETs by covalent bonds, and the fabricated device was tested using β-actin solution with concentrations from 10 -9 to 10 -3 μg/μL. Next, we examined the application of MoS 2 -FET for protein analysis in complex biological samples, and the device showed electrical signal response to human embryonic kidney cell line HEK293T in a dose-dependent manner. Furthermore, we applied this method to analyze individual liver cancer MHCC-97L cells, targeting four c

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Author

Junqing Wei; Zhihan Zhao; Kuibo Lan; Zhi Wang; Guoxuan Qin; Ruibing Chen

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Chemistry (Physical Sciences)