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Binding of a Cationic Phenazinium Dye in Anionic Liposomal Membrane: a Spectacular Modification in the Photophysics by Debosreeta Bose; Debanjana Ghosh; Priyanka Das; Agnishwar Girigoswami; Deboleena Sarkar; Nitin Chattopadhyay is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Binding of a Cationic Phenazinium Dye in Anionic Liposomal Membrane: a Spectacular Modification in the Photophysics about?
Interaction of a cationic phenazinium dye, phenosafranin (PSF), with the anionic liposomal vesicle/bilayer of dimyristoyl-l-␣-phosphatidylglycerol (DMPG) has been demonstrated using steady state and time resolved fluorescence and fluorescence anisotropy techniques. The charge transfer emission spectrum of PSF shows a dramatic modification in terms of fluorescence yield together with an appreciable hypsochromic shift in the lipid environment. The blue shift indicates a lowering in polarity inside the vesicle as compared to that in bulk water. The fluorescence and fluorescence quenching studies and micropolarity determination reveal that the cationic fluorophore has a profound binding interaction with the anionic DMPG membrane. Anisotropy study indicates the imposition of a motional restriction on the probe inside the bilayer. The electrostatic interaction between the cationic dye and the anionic lipid membrane has been argued to be the reason behind all these observations. The results could be useful in analyzing membrane organization and heterogeneity in natural membranes exploiting PSF or alike compounds as fluorescent probes.
Who reads Binding of a Cationic Phenazinium Dye in Anionic Liposomal Membrane: a Spectacular Modification in the Photophysics?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Debosreeta Bose; Debanjana Ghosh; Priyanka Das; Agnishwar Girigoswami; Deboleena Sarkar; Nitin Chattopadhyay
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-3084)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)