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A Dansyl-Derivatized Phytic Acid Analogue As A Fluorescent Substrate For Phytases - Experimental and Computational Approach by 管微石 is a document available to read on EtoBox.
This study presents the synthesis of a new fluorescent substrate, a dansyl-derivatized myo-inositol pentakisphosphate, designed for use with phytases. The substrate was engineered to enhance hydrophilicity and biocompatibility while maintaining effective interaction with the catalytic sites of two recombinant phytases, Phy-A and Phy-C. Enzymatic assays confirmed that the new substrate could be processed by both phytases with phosphate release rates comparable to natural substrates.
- Author
- 管微石
- Language
- EN