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Can I read Picoliter 1H NMR Spectroscopy on EtoBox?
Picoliter 1H NMR Spectroscopy by Kevin R. Minard; Robert A. Wind is a Chemistry article available to read on EtoBox.
What is Picoliter 1H NMR Spectroscopy about?
In this study, a 267-μm-diameter solenoid transceiver is used to acquire localized 1 H NMR spectra and the measured signal-to-noise ratio (SNR) at 500 MHz is shown to be within 20-30% of theoretical limits formulated by considering only its resistive losses. This is illustrated using a 100-μm-diameter globule of triacylglycerols (∼900 mM) that may be an oocyte precursor in young Xenopus laevis frogs and a water sample containing choline at a concentration often found in live mammalian cells (∼33 mM). In chemical shift imaging (CSI) experiments performed using a few thousand total scans, the choline methyl line is shown to have an acceptable SNR in resolved volume elements containing only 50 pL of sample, and localized spectra are resolved from just 5 pL in the Xenopus globule. These findings demonstrate the feasibility of performing 1 H NMR on picoliter-scale sample volumes in biological cells and tissues and illustrate how the achieved SNR in spectroscopic images can be predicted with reasonable accuracy at microscopic spatial resolutions.
Who reads Picoliter 1H NMR Spectroscopy?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Kevin R. Minard; Robert A. Wind
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 1090-7807)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)