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Overexpression of Human Transferrin in Two Oligodendroglial Cell Lines Enhances Their Differentiation by Pablo M. Paez; Corina I. García; Anthony T. Campagnoni; Eduardo F. Soto; Juana M. Pasquini is a Neuroscience article available to read on EtoBox.

What is Overexpression of Human Transferrin in Two Oligodendroglial Cell Lines Enhances Their Differentiation about?

## Abstract We have previously demonstrated that the addition of apotransferrin (aTf) to oligodendroglial cell (OLGc) primary cultures accelerates their maturation. Cells treated with aTf developed a multipolar morphology and displayed increased expression of mature OLGc markers. In this work, we studied the effect of Tf overexpression in two OLGc lines, N19 and N20.1. The former cells exhibit characteristics of OLGc precursors (O2A), while N20.1 cells express markers of more mature OLGcs. Using the complete cDNA of the human Tf gene, we obtained clones overexpressing Tf in both cell lines. These clones were evaluated for the expression of OLGc differentiation markers. In agreement with our previous results, we found that in the cells overexpressing Tf, there was an increased O~4~, GC, and MBP immunoreactivity. To study the myelinogenic potential of these cells, we co‐cultured N19 and N20.1 Tf‐transfected cells together with cortical neurons. There was a dramatic increase in the morphological differentiation of the OLGcs accompanied by enhanced GC and MBP expression. The OLGcs appeared to establish contact with neurites and extend their processes along them. Only two MBP isoforms w

Who reads Overexpression of Human Transferrin in Two Oligodendroglial Cell Lines Enhances Their Differentiation?

Author

Pablo M. Paez; Corina I. García; Anthony T. Campagnoni; Eduardo F. Soto; Juana M. Pasquini

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2005

Language

EN

Field

Neuroscience (Life Sciences)