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Induced Pluripotent Stem (iPS) Cells: Methods and Protocols (Methods in Molecular Biology (1357)) by Kursad Turksen (editor), Andras Nagy (editor) is a nonfiction available to read on EtoBox.

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Preface Contents Contributors The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Stu 1 Introduction 2 Materials 2.1 Mouse Embryonic Fibroblast Preparation 2.1.1 Mice 2.1.2 MEF Medium 2.1.3 MEF Isolation 2.1.4 MEF Passage 2.1.5 Cell Counting 2.1.6 Preparation of Frozen MEF Stocks 2.2 Primary (1o) Reprogramming 2.2.1 Gelatin Coating 2.2.2 MEF Defrost 2.2.3 MEF Seeding for PB Transfection 2.2.4 PB Transfection 2.2.5 Mouse iPSC Culture Medium 2.2.6 Doxycycline Solution 2.2.7 Alkaline Phosphatase Staining 2.2.8 Surface Marker Analysis: Flow Cytometry 2.2.9 Whole-Well Fluorescence Microscopy Imaging 2.3 PB-iPSC Clone Screening/Validation for Secondary (2o) Reprogramming Application 2.3.1 Cells 2.3.2 PB-iPSC Colony Picking 2.3.3 PB-iPSC Expansion 2.3.4 Nanog-GFP Reporter Analysis by Flow Cytometry 2.3.5 PB-iPSC Freezing 2.3.6 Splinkerette PCR Screening 2.3.7 Southern Blotting 2.3.8 Preparation of Candidate PB-iPSC Lines 3 Methods 3.1 The PB Vector 1o Reprogramming System 3.2 Mouse Embryonic Fibroblast Preparation 3.2.1 Isolating MEFs 3.2.2 Passaging MEFs 3.2.3 Freezing MEFs 3.3 Primary (1o) Reprogramming of MEFs with PB Transposons 3.3.1 Defrosting MEFs (-d4)

Who reads Induced Pluripotent Stem (iPS) Cells: Methods and Protocols (Methods in Molecular Biology (1357))?

Author

Kursad Turksen (editor), Andras Nagy (editor)

Publisher

Humana Press, Springer [distributor

Published

2015

Language

EN

ISBN

9781493930548

Category

nonfiction

Subjects

Science, Biology, Stem