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Can I read The Buffy Coat Method: a Tool for Detection of Blood Parasites Without Staining Procedures on EtoBox?
The Buffy Coat Method: a Tool for Detection of Blood Parasites Without Staining Procedures by Chagas, Carolina R. F.; BinkienÄ, Rasa; IlgÅ«nas, Mikas; Iezhova, Tatjana; ValkiÅ«nas, Gediminas is a Medicine article available to read on EtoBox.
What is The Buffy Coat Method: a Tool for Detection of Blood Parasites Without Staining Procedures about?
## Background Blood parasites belonging to the Apicomplexa, Trypanosomatidae and Filarioidea are widespread in birds and have been studied extensively. Microscopical examination (ME) of stained blood films remains the gold standard method for the detection of these infections in birds, particularly because co-infections predominate in wildlife. None of the available molecular tools can detect all co-infections at the same time, but ME provides opportunities for this to be achieved. However, fixation, drying and staining of blood films as well as their ME are relatively time-consuming. This limits the detection of infected hosts during fieldwork when captured animals should be released soon after sampling. It is an obstacle for quick selection of donor hosts for parasite experimental, histological and other investigations in the field. This study modified, tested and described the buffy coat method (BCM) for quick diagnostics (~ 20 min/sample) of avian blood parasites. ## Methods Blood of 345 birds belonging to 42 species was collected, and each sample was examined using ME of stained blood films and the buffy coat, which was examined after centrifugation in capillary tubes and afte
Who reads The Buffy Coat Method: a Tool for Detection of Blood Parasites Without Staining Procedures?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Chagas, Carolina R. F.; BinkienÄ, Rasa; IlgÅ«nas, Mikas; Iezhova, Tatjana; ValkiÅ«nas, Gediminas
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2008-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Research Square (ISSN 1756-3305)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)