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Differential homogenization and amplification of two satellite DNAs in the genus Cucurbita (Cucurbitaceae) by Klaus King; Jürgen Jobst; Vera Hemleben is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Differential homogenization and amplification of two satellite DNAs in the genus Cucurbita (Cucurbitaceae) about?

Two different satellite DNAs exist in the genus Cucurbita which are different with respect to repeat length (350 bp and 170 bp), array size, and sequence homogenization. Whereas the 350-bp satellite DNA is prominent and very homogeneous in all species investigated except for C. maxima and C. lundelliana, the 170bp satellite is rather evenly distributed in all species. In C. maxima and C. lundelliana the 350-bp satellite is present only in small amounts, but detectable by the sensitive PCR method. These repeats are also very homogeneous, reflecting a silent stage of satellite DNA. In contrast, the 170-bp satellite DNA is intra-and interspeciflcally heterogeneous. It is striking that the species with no detectable amount of 350-bp satellite contain 170-bp satellite DNA clusters with the highest degree of homogeneity. The evolution of satellite DNA repeats within cultivated and wild species in the genus Cucurbita is elucidated using the sequence data of both satellite DNAs from all species investigated. The value of satellite DNA for phylogenetic analysis between closely related species is discussed.

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Author

Klaus King; Jürgen Jobst; Vera Hemleben

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0022-2844)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)