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Genetic organization of sulphur‐controlled aryl desulphonation in Pseudomonas putida S‐313 by Paul Vermeij; Claudia Wietek; Antje Kahnert; Thomas Wüest; Michael A. Kertesz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Genetic organization of sulphur‐controlled aryl desulphonation in Pseudomonas putida S‐313 about?

__Pseudomonas putida__ S‐313 is able to desulphonate a broad range of aromatic sulphonates to provide sulphur for growth by monooxygenolytic cleavage to yield the corresponding phenol. After miniTn__5__ transposon mutagenesis of this strain, 11 mutants were isolated that were no longer able to utilize benzenesulphonate as a sulphur source. Three of these mutants were defective in the utilization of all aromatic sulphonates tested, but they grew normally with other sulphur sources. These strains contained independent insertions in the novel 4.2 kb __asfRABC__ gene cluster, encoding a putative reductase (AsfA), a ferredoxin (AsfB), a putative periplasmic binding protein (AsfC), which was localized to the periplasm using alkaline phosphatase fusions, and a divergently oriented fourth gene, __asfR__, that encoded a LysR‐type regulator protein. A further mutant was interrupted in the __ssu__ locus, which includes the gene for a putative desulphonative monooxygenase. Transformation of __Pseudomonas aeruginosa__ with the __asfRAB__ genes was sufficient to allow arylsulphonate utilization by this species, which does not normally use these compounds, suggesting that the AsfAB proteins may c

Who reads Genetic organization of sulphur‐controlled aryl desulphonation in Pseudomonas putida S‐313?

Author

Paul Vermeij; Claudia Wietek; Antje Kahnert; Thomas Wüest; Michael A. Kertesz

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)