Can I read Monoclonal antibodies against type II rat brain protein kinase C. on EtoBox?

Monoclonal antibodies against type II rat brain protein kinase C. by H Nakabayashi; K P Huang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Monoclonal antibodies against type II rat brain protein kinase C. about?

Monoclonal antibodies (8/1, 10/10, and 25/3) against rat brain type II protein kinase C were used for the immunochemical characterization of this kinase. These antibodies immunoprecipitated the type II protein kinase C in a dose-dependent manner but did neither to the type I nor III isozyme. Immunoblot analysis of the tryptic fragments from protein kinase C revealed that all three antibodies recognized the 27-38-kDa fragments, the phospholipid/phorbol ester-binding domain, but not the 45-48-kDa fragments, the kinase catalytic domain. The immune complexes of the kinase and the antibodies retained 70-80% of the kinase activity which was dependent on Ca2+ and phosphatidylserine and further activated by diacylglycerol or tumor-promoting phorbol ester. With antibody 8/1, the kinetic parameters with respect to Km for ATP and histone and K alpha for phosphatidylserine and phorbol 12,13-dibutyrate were not significantly influenced. However, the antibody causes variable effects on the K alpha for Ca2+ under different assay conditions. When determined in the presence of phosphatidylserine, the K alpha for Ca2+ was reduced by an order of magnitude (37 +/- 8 to 2.0 +/- 1.8 microM); in the pres

Who reads Monoclonal antibodies against type II rat brain protein kinase C.?

Author

H Nakabayashi; K P Huang

Publisher

Elsevier BV

Published

1988

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)