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Analysis of the molecular mechanisms of targeted anti-caries DNA plasmid enhancing antibody responses by gene arrays by Qing-An Xu; Fei Yu; Mingwen Fan; Quan Xing; Gaoxia Liu; Chang Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background The cytotoxic T lymphocyte antigen 4 (CTLA4) represents an attractive ligand for use in the targeting of antigens to dendritic cells (DCs). Studies have shown that CTLA4 targeted DNA vaccines induced accelerated and increased antibody responses compared to nontargeted vaccines. However, little is known about the molecular events on DCs after transfection with targeted DNA vaccines. ## Methods Here we constructed a green fluorescent protein (GFP)‐labeled targeted anti‐caries plasmid pMGJGLU/GFP and a GFP‐labeled nontargeted anti‐caries plasmid pCDGLU/GFP, compared the antibody responses they induced in mice, and investigated the gene expression profiles of DCs transfected with pMGJGLU/GFP and pCDGLU/GFP by gene array analysis. ## Results The data obtained showed that pMGJGLU/GFP induced accelerated and increased serum antibody responses in mice compared to pCDGLU/GFP. Overall, the expression levels of 28 genes were up‐regulated in pMGJGLU/GFP transfected DCs compared to pCDGLU/GFP transfected DCs by gene array analysis. Real‐time reverse transcriptase‐polymerase chain reaction analysis on pMGJGLU/GFP transfected DCs and pCDGLU/GFP transfected DCs confirmed

Who reads Analysis of the molecular mechanisms of targeted anti-caries DNA plasmid enhancing antibody responses by gene arrays?

Author

Qing-An Xu; Fei Yu; Mingwen Fan; Quan Xing; Gaoxia Liu; Chang Liu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)