About this Engineering article
Application of Deep Eutectic Solvents for the Extraction of Phenolic Compounds from Extra‐virgin Olive Oil by Chiara Fanali; Susanna Della Posta; Laura Dugo; Marina Russo; Alessandra Gentili; Luigi Mondello; Laura De Gara is a Engineering article available to read on EtoBox.
## Abstract A HPLC–DAD/ESI–MS method has been developed and validated for the analysis of the most representative phenolic compounds in extra‐virgin olive oil (EVOO) samples using a green extraction approach based on deep eutectic solvents (DESs) at room temperature. We examined ten DESs based on choline chloride and betaine in combination with different hydrogen bond donors comprising six alcohols, two organic acids, and one urea. Five phenolic compounds, belonging to the classes of secoiridoids and phenolic alcohols, were selected for the evaluation of extraction efficiency. A betaine‐based DES with glycerol (molar ratio 1:2) was found to be the most effective for extracting phenolic compounds as compared to a conventional solvent. The optimization of the extraction method involved the study of the quantity of water to be added to the DES and evaluation of the sample‐to‐solvent ratio optimal condition. Thirty percent of water added to DES and sample to solvent ratio 1:1 (w/v) were selected as the best conditions. The chromatographic method was validated by studying LOD, LOQ, intraday and interday retention time precision, and linearity range. Recovery values obtained spiking seed
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Chiara Fanali; Susanna Della Posta; Laura Dugo; Marina Russo; Alessandra Gentili; Luigi Mondello; Laura De Gara
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0173-0835)
- Published
- 2020
- Language
- EN
- Field
- Engineering (Physical Sciences)