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Splenic aspiration is the most reliable method for diagnosing visceral leishmaniasis. It has a positivity rate of 95-98% for detecting leishmania amastigotes. It is simple, cheap, and can be performed in remote areas without expensive equipment. Bone marrow aspiration has a lower positivity rate of 64-86% for detection. Examination of various clinical samples like spleen, bone marrow, lymph nodes, and buffy coat are discussed for detection of leishmania amastigotes microscopically after staining.

Author
Megbaru
Language
EN

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