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Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection by S AHMED; N KISHIKAWA; K OHYAMA; M WADA; K NAKASHIMA; N KURODA is a Chemistry article available to read on EtoBox.

A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in the presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-μL was sufficient. Besides, it can detect accurately the low plasma concentrations. The detection limits (signal-to-noise ratio=3) were 4.5 and 3.8fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7–102.4% and the inter- and intra-assay R

Author

S AHMED; N KISHIKAWA; K OHYAMA; M WADA; K NAKASHIMA; N KURODA

Publisher

Elsevier BV

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)