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Impairment of branchial and coronary blood flow reduces reproductive fitness, but not cardiac performance in paternal small ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Study animals, study sites and identification of nest sites 2 2.2 Fish capture and surgical procedures 3 2.3 Behavioural monitoring and assessment 3 2.4 Fish recapture, assessment of maximum heart rate and logger removal 3 2.5 Monitoring of egg development 4 2.6 Programming of heart rate loggers and heart rate analyses 4 2.7 Statistics 4 3 Results 5 3.1 Effects of branchial/coronary ligation on reproductive fitness in parental bass 5 3.2 Effects of branchial/coronary ligation on parental care behaviours in paternal smallmouth bass 5 3.3 Effects of branchial/coronary ligation on parental care behaviours in paternal bass instrumented with heart rate loggers 5 3.4 Effects of branchial/coronary ligation on heart rate performance in parental bass instrumented with heart rate loggers 5 4 Discussion 5 4.1 Conclusions and future perspectives 7 Author contributions 8 Funding 9 Declaration of Competing Interest 9 Acknowledgements 9 Appendix A Supplementary data 9 References 9

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EN

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nonfiction