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This document provides instructions for analyzing DNA fragments through restriction digestion and agarose gel electrophoresis. Students will digest the plasmid pCMV-GFP with restriction enzymes EcoRI, EcoRV, and MluI. They will then load the digested samples into a 1% agarose gel and perform electrophoresis to separate the fragments by size. This will allow them to determine the restriction map of the plasmid by analyzing the number and sizes of fragments produced by each enzyme.

Author

Lloaana 12

Language

EN