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Onset of force development as a marker of thrombin generation in whole blood: the thrombin generation time (TGT) by M. E. Carr Jr; E. J. Martin; J. G. Kuhn; B. D. Spiess is a Medicine article available to read on EtoBox.

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Prothrombin activation requires the direct interplay of activated platelets and plasma clotting factors. Once formed, thrombin causes profound, irreversible activation of platelets and reinforces the platelet plug via ®brin formation. Delayed or de®cient thrombin production increases bleeding risk. Commonly employed coagulation assays, the prothrombin and partial thromboplastin times, use clot formation as a surrogate marker of thrombin generation. These assays routinely utilize platelet-poor plasma and completely miss the effects of platelets. Other markers of thrombin generation, prothrombin fragment 1 2 (F1 2) and thrombin±antithrombin complex, are typically measured after the fact. We report a simple assay, which employs the onset of platelet contractile force (PCF) as a surrogate marker of thrombin generation. PCF generation occurs concomitant with the burst of F1 2 release. The time between assay start and PCF onset is termed the thrombin generation time (TGT). TGT is prolonged in clotting factor de®ciencies and in the presence of direct and indirect thrombin inhibitors. TGT shortens to normal with clotting factor replacement and shortens with administration of recombinant fa

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Author

M. E. Carr Jr; E. J. Martin; J. G. Kuhn; B. D. Spiess

Publisher

Elsevier BV

Published

2003

Language

EN

Field

Medicine (Health Sciences)