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Spermatozoal Transcripts Associated with Oxidative Stress and Mitochondrial Membrane Potential Differ Between High‐ and Low‐fertile Crossbred Bulls by Kaustubh Kishor Saraf; Arumugam Kumaresan; Manish Kumar Sinha; Tirtha Kumar Datta is a Medicine article available to read on EtoBox.
What is Spermatozoal Transcripts Associated with Oxidative Stress and Mitochondrial Membrane Potential Differ Between High‐ and Low‐fertile Crossbred Bulls about?
The presence of various forms of RNAs having roles in fertilisation and early embryonic development is well documented in mammalian spermatozoa. In the present study, using Agilent microarray platform, we compared sperm mRNA expression profiles between high- and low-fertile crossbred bulls with normal semen parameters. Microarray data acquisition and analysis were performed using GeneSpring GX version software, wherein spermatozoa from high-fertile bulls were kept as control while spermatozoa from low-fertile bulls were considered as treatment group. A total of 6,238 transcripts were detected in crossbred bull spermatozoa; 559 transcripts (>1.5-fold) were differentially regulated between high- and low-fertile bulls. Functional annotation has categorised these transcripts into biological process, cellular, and molecular functions. It was observed that transcripts associated with oxidation reduction process (p = .003), mitochondrial membrane potential (p = .03), were significantly down-regulated while transcripts associated with apoptosis (p = .04) were up-regulated in low-fertile spermatozoa. The dysregulated genes were involved in important cellular pathways including oxidative pho
Who reads Spermatozoal Transcripts Associated with Oxidative Stress and Mitochondrial Membrane Potential Differ Between High‐ and Low‐fertile Crossbred Bulls?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Kaustubh Kishor Saraf; Arumugam Kumaresan; Manish Kumar Sinha; Tirtha Kumar Datta
- Publisher
- Hindawi Limited
- Published
- 2021
- Language
- EN
- Field
- Medicine (Health Sciences)