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SNAP-25 requirement for dendritic growth of hippocampal neurons by Gisela Grosse; Johannes Grosse; Rosemarie Tapp; Jörg Kuchinke; Martin Gorsleben; Ingmar Fetter; Barbara Höhne-Zell; Manfred Gratzl; Mathias Bergmann is a Neuroscience article available to read on EtoBox.

What is SNAP-25 requirement for dendritic growth of hippocampal neurons about?

Structure and dimension of the dendritic arbor are important determinants of information processing by the nerve cell, but mechanisms and molecules involved in dendritic growth are essentially unknown. We investigated early mechanisms of dendritic growth using mouse fetal hippocampal neurons in primary culture, which form processes during the first week in vitro. We detected a key component of regulated exocytosis, SNAP-25 (synaptosomal associated protein of 25 kDa), in axons and axonal terminals as well as in dendrites identified by the occurrence of the dendritic markers transferrin receptor and MAP2. Selective inactivation of SNAP-25 by botulinum neurotoxin A (BoNTA) resulted in inhibition of axonal growth and of vesicle recycling in axonal terminals. In addition, dendritic growth of hippocampal pyramidal and granule neurons was significantly inhibited by BoNTA. In contrast, cleavage of synaptobrevin by tetanus toxin had an effect on neither axonal nor dendritic growth. Our observations indicate that SNAP-25, but not synaptobrevin, is involved in constitutive axonal growth and dendrite formation by hippocampal neurons.

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Author

Gisela Grosse; Johannes Grosse; Rosemarie Tapp; Jörg Kuchinke; Martin Gorsleben; Ingmar Fetter; Barbara Höhne-Zell; Manfred Gratzl; Mathias Bergmann

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1999

Language

EN

Field

Neuroscience (Life Sciences)