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Effect of nutritional factors on cellulase enzyme and microbial protein production by Aspergillus terreus and its evaluation by S. K. Garg; S. Neelakantan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The biomass yield, cellulolytic activity, and protein recovery using __Aspergillus terreus__ GN1 with alkali‐treated sugarcane bagasse was studied using different levels (250–600 mg of N/L of broth) of organic and inorganic nitrogen sources. e.g., cattle urine, urea, cornsteep liquor, ammonium sulfate, ammonium nitrate, ammonium iron sulfate, ammonium chloride, and sodium nitrate. Among different levels of alkali‐treated bagasse substrate concentrations (0.5–4.0% w/v) tested, 1.0% substrate yielded the highest crude protein content, protein recovery, and cellulolytic activity. The biomass recovery with 1.0% substrate ranged from 290–380 mg/500 mg bagasse substrate in a 50‐mL broth with a nitrogen level of 250–600 mg of N/L in all the sources except ammonium iron sulfate, which yielded 402–439 mg/500 mg bagasse substrate. However, crude protein content of biomass obtained with an ammonium iron sulfate nitrogen source was the lowest. Cornsteep liquor nitrogen source at the rate of 600 mg of N/L yielded the maximum crude protein of 32.9%, protein recovery of 22.2 g/100 g of bagasse, and carboxymethyl cellulase and filter paper enzyme activities of 1.1 and 0.09 units/mL, am

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Author

S. K. Garg; S. Neelakantan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1982

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)