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What is Understanding Spectrophotometers about?

A colorimeter or spectrophotometer uses the transmission of light through a solution to determine the concentration of a solute. A spectrophotometer separates light into wavelengths using a prism, while a colorimeter uses filters. Both measure the amount of light transmitted through a sample. The amount of light absorbed relates to the concentration - diluting a solution reduces its intensity and the voltage measured. The Beer-Lambert law states that absorbance, the negative log of transmittance, is directl

Author

Anura Bandara

Language

EN