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This document presents a novel method for tagging endogenous genes using promoter trapping and short homology arms, significantly improving the efficiency and specificity of genome editing via homology-directed repair (HDR). The method achieves tagging efficiencies of 50% to 100% with a 1.4 kb promoterless GFP reporter, validated across various cell lines, including human induced pluripotent and hematopoietic stem cells. This approach has broad applications in genome engineering, DNA cloning, and immune cel

Author

권준철

Language

EN