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Can I read Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry on EtoBox?
Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry by Jennifer A. Paquette; Steven W. Kumpf; Randal D. Streck; Jason J. Thomson; Robert E. Chapin; Donald B. Stedman is a Medicine article available to read on EtoBox.
What is Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry about?
## Abstract **BACKGROUND:** The European Centre for the Validation of Alternative Methods (ECVAM) designed the Embryonic Stem Cell Test (EST) as a tool for classifying developmentally toxic compounds. An in vitro tool to assess developmental toxicity would be of great value to the pharmaceutical industry to help with toxicity‐associated attrition. **METHODS:** ECVAM's EST protocol was used, but employing a different mouse embryonic stem cell (ESC) line and an alternative differentiation medium. A subset of the compounds used to validate the EST assay along with a number of in‐house pharmaceutical compounds plus marketed pharmaceutical compounds were used to assess the EST performance with receptor‐mediated compounds. **RESULTS:** Our results with ECVAM compounds mirrored ECVAM's. Compounds that were developmentally toxic in vivo were classified by the EST as moderate risk. Overall, the accuracy was 75% with the current set of data and the predictivity of low‐, moderate‐, and high‐risk compounds was 90, 71, and 60% while the precision was 59, 86, and 100%, respectively. Interestingly, a number of the non‐developmentally toxic compounds had values for the 3T3 IC~50~ values, which wer
Who reads Assessment of the Embryonic Stem Cell Test and application and use in the pharmaceutical industry?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Jennifer A. Paquette; Steven W. Kumpf; Randal D. Streck; Jason J. Thomson; Robert E. Chapin; Donald B. Stedman
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1542-9733)
- Published
- 2008
- Language
- EN
- Field
- Medicine (Health Sciences)