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Covalent Grafting of Fibronectin onto Plasma‐Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity by Karine Vallières; Éric Petitclerc; Gaétan Laroche is a Materials Science article available to read on EtoBox.

What is Covalent Grafting of Fibronectin onto Plasma‐Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity about?

## Abstract Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(__p__‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy. magnified image

Who reads Covalent Grafting of Fibronectin onto Plasma‐Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity?

Author

Karine Vallières; Éric Petitclerc; Gaétan Laroche

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1616-5187)

Published

2007

Language

EN

Field

Materials Science (Physical Sciences)