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Spatiotemporal control of subcellular O-GlcNAc signaling using Opto-OGT by Qunxiang Ong & Ler Ting Rachel Lim & Cameron Goh & Yilie Liao & Sher En Chan & Crystal Jing Yi Lim & Valerie Kam & Jerome Yap & Tiffany Tseng & Reina Desrouleaux & Loo Chien Wang & Siok Ghee Ler & Siew Lan Lim & Sun-Yee Kim & Radoslaw M. Sobota & Anton... is a book available to read on EtoBox.

What is Spatiotemporal control of subcellular O-GlcNAc signaling using Opto-OGT about?

Nature Chemical Biology, doi:10.1038/s41589-024-01770-7The post-translational modifcation of intracellular proteins through O-linked β-N-acetylglucosamine (O-GlcNAc) is a conserved regulatory mechanism in multicellular organisms. Catalyzed by O-GlcNAc transferase (OGT), this dynamic modifcation has an essential role in signal transduction, gene expression, organelle function and systemic physiology. Here, we present Opto-OGT, an optogenetic probe that allows for precise spatiotemporal control of OGT activity through light stimulation. By fusing a photosensitive cryptochrome protein to OGT, Opto-OGT can be robustly and reversibly activated with high temporal resolution by blue light and exhibits minimal background activity without illumination. Transient activation of Opto-OGT results in mTORC activation and AMPK suppression, which recapitulate nutrient-sensing signaling. Furthermore, Opto-OGT can be customized to localize to specifc subcellular sites. By targeting OGT to the plasma membrane, we demonstrate the downregulation of site-specifc AKT phosphorylation and signaling outputs in response to insulin stimulation. Thus, Opto-OGT is a powerful tool for defning the role of O-GlcNA

Author

Qunxiang Ong & Ler Ting Rachel Lim & Cameron Goh & Yilie Liao & Sher En Chan & Crystal Jing Yi Lim & Valerie Kam & Jerome Yap & Tiffany Tseng & Reina Desrouleaux & Loo Chien Wang & Siok Ghee Ler & Siew Lan Lim & Sun-Yee Kim & Radoslaw M. Sobota & Anton...

Publisher

x

Published

2025

Language

EN