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Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54 by Gina Lisignoli; Sandra Cristino; Anna Piacentini; Carola Cavallo; Arnold I. Caplan; Andrea Facchini is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54 about?
## Abstract Hyaluronan (HA), in the bone marrow stroma, is the major non‐protein glycosaminoglycan component of extracellular matrix (ECM) involved in cell positioning, proliferation, differentiation as well as in receptor‐mediated changes in gene expression. Repair of bone and regeneration of bone marrow is dependent on ECM, inflammatory factors, like chemokines and degradative factors, like metalloproteinases. We analyzed the interaction between human mesenchymal stem cells (h‐MSCs) and a three‐dimensional (3‐D) HA‐based scaffold in vitro. The expression of CXC chemokines/receptors, CXCL8 (IL‐8)/CXCR1‐2, CXCL10 (IP‐10)/CXCR3, CXCL12 (SDF‐1)/CXCR4, and CXCL13 (BCA‐1)/CXCR5, and metalloproteinases/inhibitors MMP‐1, MMP‐3, MMP‐13/TIMP‐1 were evaluated in h‐MSCs grown on plastic or on HA‐based scaffold by Real‐time PCR, ELISA, and immunocytochemical techniques. Moreover, the expression of two HA receptors, CD44 and CD54, was analyzed. We found both at mRNA and protein levels that HA‐based scaffold induced the expression of CXCR4, CXCL13, and MMP‐3 and downmodulated the expression of CXCL12, CXCR5, MMP‐13, and TIMP‐1 while HA‐based scaffold induced CD54 expression but not CD44. We fou
Who reads Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gina Lisignoli; Sandra Cristino; Anna Piacentini; Carola Cavallo; Arnold I. Caplan; Andrea Facchini
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)