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Primitive nucleobases @ sodium 2-Ketooctanoate vesicles with high salt resistance 1 1 Introduction 1 2 Experimental section 2 2.1 Materials 2 2.2 Preparation of PNB@SKO solutions 2 2.3 Determination of salt-resistance for vesicle systems 2 2.4 Measurements 2 2.4.1 Negative-staining transmission electron microscope (NS-TEM) 2 2.4.2 Equilibrium surface tension 2 2.4.3 Dynamic light scattering (DLS) 2 2.4.4 Small angle X-ray scattering (SAXS) 2 2.4.5 Fourier transform infrared (FT-IR) spectroscopy measurements 2 2.4.6 Molecular dynamics simulation 2 2.4.7 Quantum chemistry calculation 2 3 Results and discussions 3 3.1 Formation of PNB@SKO vesicles 3 3.2 Binding rate of PNB to SKO vesicle 4 3.3 Effect of PNB on the CVC of SKO in water 4 3.4 Possible mechanism of PNB@SKO vesicle formation 4 3.5 Salt-resistance of PNB@SKO vesicles 6 4 Conclusion 7 CRediT authorship contribution statement 8 Declaration of Competing Interest 8 Acknowledgement 8 Appendix A Supplementary material 8 References 8

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nonfiction