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MicroRNA-4487 regulates vascular smooth muscle cell proliferation, migration and apoptosis by targeting RAS p21 protein activator 1 by Xiao Liang; Miaoyang Hu; Wei Yuan; Yang Liu; Jingjin Li; Chuan Bai; Zuyi Yuan is a Medicine article available to read on EtoBox.
What is MicroRNA-4487 regulates vascular smooth muscle cell proliferation, migration and apoptosis by targeting RAS p21 protein activator 1 about?
## Background: Dysregulation of microrna (mirna) is involved in the pathogenesis of a variety of diseases, including atherosclerosis (as). however, the role of mirna-4487 (mir-4487) in the development of as is not fully clarified. this study is intended to investigate the regulatory effects of mir-4487 on the proliferation, migration and apoptosis of vascular smooth muscle cells (vsmcs) and the related mechanisms. ## Methods: Oxidized low-density lipoprotein (ox-ldl) was employed to induce the dysfunction of vsmcs. subsequently, mir-4487 expression was detected by quantitative real-time pcr. afterward, the expression levels of ras p21 protein activator 1 (rasa1) and apoptosis-related proteins (bcl-2, bax, cleaved caspase 3, cleaved caspase 9) were detected by western blotting. the proliferation, migration and apoptosis of vsmcs were then detected by cck-8, brdu, transwell and flow cytometry assays, respectively. moreover, a dual-luciferase reporter gene assay was executed to verify the targeting between mir-4487 to the rasa1 3'-untranslated region (3'-utr). ## Results: Ox-ldl treatment increased mir-4487 expression and decreased rasa1 expression in vsmcs. additionally, ox-ldl treat
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- Author
- Xiao Liang; Miaoyang Hu; Wei Yuan; Yang Liu; Jingjin Li; Chuan Bai; Zuyi Yuan
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)