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Redox-regulation of Erk1/2-directed phosphatase by reactive oxygen species: Role in signaling TPA-induced growth arrest in ML-1 cells by Kassim Traore; Rajni Sharma; Rajesh K. Thimmulappa; Walter H. Watson; Shyam Biswal; Michael A. Trush is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Redox-regulation of Erk1/2-directed phosphatase by reactive oxygen species: Role in signaling TPA-induced growth arrest in ML-1 cells about?
## Abstract Extracellular signal‐regulated kinase (Erk)1/2 activity signals myeloid cell differentiation induced by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA). Previously, we reported that Erk1/2 activation (phosphorylation) induced by TPA required reactive oxygen species (ROS) as a second messenger. Here, we hypothesized that ROS generated in response to TPA inhibit Erk1/2‐directed phosphatase activity, which leads to an increase phosphorylation of Erk1/2 to signal p21^WAF1/Cip1^‐mediated growth arrest in ML‐1 cells. Incubation of ML‐1 cells with TPA resulted in a marked accumulation of phosphorylated Erk1/2, and is subsequent to H~2~O~2~ generation. Interestingly, post‐TPA‐treatment with N‐acetylcysteine (NAC) stimulated a marked and a rapid dephosphorylation of Erk1/2, suggesting a regeneration of Erk1/2‐directed phospahatase activity by NAC. ROS generation in ML‐1 cells induced by TPA was suggested to occur in the mitochondrial electron transport chain (METC) based on the following observations: (i) undifferentiated ML‐1 cells not only lack p67‐phox and but also express a low level of p47‐phox key components required for NADPH oxidase enzymatic activity, (ii) pretreatment with
Who reads Redox-regulation of Erk1/2-directed phosphatase by reactive oxygen species: Role in signaling TPA-induced growth arrest in ML-1 cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kassim Traore; Rajni Sharma; Rajesh K. Thimmulappa; Walter H. Watson; Shyam Biswal; Michael A. Trush
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)