Can I read General Reversed-phase High-performance Liquid Chromatography Procedure for the Analysis of Oral Contraceptive Formulations on EtoBox?

General Reversed-phase High-performance Liquid Chromatography Procedure for the Analysis of Oral Contraceptive Formulations by G. Carignan; B.A. Lodge; W. Skakum is a Chemistry article available to read on EtoBox.

What is General Reversed-phase High-performance Liquid Chromatography Procedure for the Analysis of Oral Contraceptive Formulations about?

Oral contraceptive formulations are for the most part a mixture of two types of biologically active steroids, an estrogen and a progestin; formulations containing only a progestin are also available. They present an interesting problem to the analyst, as the concentration of active ingredient in various formulations may vary over the range 25 pug-5.0 mg per tablet. Furthermore, there are considerable differences between the UV maxima (end absorption to 280 nm) as well as the absorption coefficients of the various drugs used in these preparations. Oral contraceptive formulations have been quantitatively assayed by a large number of procedures. These include colorimetry1-3, UV determination after acidic conversion to a UV absorbing compound4, UV determination of progestin having a natural UV chromophore4, and fluorometry of estrogens5T6, with and without prior separation of active ingredients, quantitative thin-layer chromatography (TLC)', gas-liquid chromatography (GLC)\*-l O and high-performance liquid chromatography (HPLC)"-13. All these procedures are for one particular ingredient or formulation and cannot be used to analyze all possible combinations of progestins and estrogens.

Who reads General Reversed-phase High-performance Liquid Chromatography Procedure for the Analysis of Oral Contraceptive Formulations?

Author

G. Carignan; B.A. Lodge; W. Skakum

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1984

Language

EN

Field

Chemistry (Physical Sciences)