Opening book details…
Can I read An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency on EtoBox?
An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency by Susan T. Mashiyama; Christine M. Hansen; Esther Roitman; Stella Sarmiento; James E. Leklem; Terry D. Shultz; Bruce N. Ames is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency about?
Improvements are made to our gas-chromatography-mass-spectrometry-based assay for quantifying low levels of DNA-uracil. Folate deficiency leads to increased deoxyuridine monophosphate/thymidylate (dUMP/dTMP) ratios and uracil misincorporation into DNA, which may increase cancer risk. Vitamin B6 (B6) deficiency might also result in increased DNA-uracil because B6 is a cofactor for serine hydroxymethyltransferase, which catalyzes the methylation of tetrahydrofolate (THF) to methylene-THF, the folate form that is required to convert dUMP to dTMP. However, the low baseline levels of DNA-uracil in healthy human lymphocytes are difficult to measure accurately. This version of the assay (Uracil assay V3) has an approximately 10-fold increase in signal strength over the previous method and a 10-fold lower detection limit (0.2 pg uracil). Five micrograms of DNA, the amount in about 1 ml of human blood, is a suitable amount for this assay. Using this improved assay, DNA-uracil was measured in lymphocytes from 12 healthy smoking or nonsmoking young men and women who consumed a B6-restricted diet (0.7 mg B6/day, or %half the recommended dietary allowance) for 28 days. DNA-uracil concentration
Who reads An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Susan T. Mashiyama; Christine M. Hansen; Esther Roitman; Stella Sarmiento; James E. Leklem; Terry D. Shultz; Bruce N. Ames
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)