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Can I read Driving Forces of the Complex Formation Between Highly Charged Disordered Proteins on EtoBox?
Driving Forces of the Complex Formation Between Highly Charged Disordered Proteins by Aritra Chowdhury; Alessandro Borgia; Souradeep Ghosh; Andrea Sottini; Soumik Mitra; Rohan S. Eapen; Madeleine B. Borgia; Tianjin Yang; Nicola Galvanetto; Miloš T. Ivanović; Paweł Łukijańczuk; Ruijing Zhu; Daniel Nettels; Arindam Kundagrami; Benjamin Schuler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Driving Forces of the Complex Formation Between Highly Charged Disordered Proteins about?
## Significance Charged disordered proteins are abundant in the cell, especially in the nucleus, and oppositely charged molecules can form high-affinity disordered complexes. Such interactions can exhibit surprising behavior, such as increased affinity with increasing temperature, but a detailed characterization of the underlying thermodynamic driving forces has been lacking. Combining single-molecule FRET (Förster resonance energy transfer), calorimetry, and analytical polymer theory, we dissect the thermodynamics of the disordered high-affinity complex between two highly charged disordered proteins and find that the release of counterions is an essential entropic contribution to binding. Our results also demonstrate the formation of higher-order complexes at high protein concentrations, and provide a quantitative experimental and theoretical framework for elucidating the interactions of charged disordered biomolecules and synthetic polyelectrolytes.
Who reads Driving Forces of the Complex Formation Between Highly Charged Disordered Proteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Aritra Chowdhury; Alessandro Borgia; Souradeep Ghosh; Andrea Sottini; Soumik Mitra; Rohan S. Eapen; Madeleine B. Borgia; Tianjin Yang; Nicola Galvanetto; Miloš T. Ivanović; Paweł Łukijańczuk; Ruijing Zhu; Daniel Nettels; Arindam Kundagrami; Benjamin Schuler
- Publisher
- National Academy of Sciences
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)