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Merkel Cell Polyomavirus (MCPyV) in the Context of Immunosuppression: Genetic Analysis of Noncoding Control Region (NCCR) Variability among a HIV-1-Positive Population by Prezioso, Carla; Obregon, Francisco; Ambroselli, Donatella; Petrolo, Sara; Checconi, Paola; Rodio, Donatella Maria; Coppola, Luigi; Nardi, Angelo; Vito, Corrado de; Sarmati, Loredana; Andreoni, Massimo; Palamara, Anna Teresa; Ciotti, Marco; Pietropaolo, Valeria is a Medicine article available to read on EtoBox.

What is Merkel Cell Polyomavirus (MCPyV) in the Context of Immunosuppression: Genetic Analysis of Noncoding Control Region (NCCR) Variability among a HIV-1-Positive Population about?

Background: Since limited data are available about the prevalence of Merkel cell polyomavirus (MCPyV) and the genetic variability of its noncoding control region (NCCR) in the context of immunosuppression, this study aimed to investigate the distribution of MCPyV in anatomical sites other than the skin and the behavior of NCCR among an HIV-1-positive population. Methods: Urine, plasma, and rectal swabs specimens from a cohort of 66 HIV-1-positive patients were collected and subjected to quantitative real-time polymerase chain reaction (qPCR) for MCPyV DNA detection. MCPyV-positive samples were amplified by nested PCR targeting the NCCR, and NCCRs alignment was carried out to evaluate the occurrence of mutations and to identify putative binding sites for cellular factors. Results: MCPyV DNA was detected in 10/66 urine, in 7/66 plasma, and in 23/66 rectal samples, with a median value of 5 × 10^2^ copies/mL, 1.5 × 10^2^ copies/mL, and 2.3 × 10^3^ copies/mL, respectively. NCCR sequence analysis revealed a high degree of homology with the MCC350 reference strain in urine, whereas transitions, transversions, and single or double deletions were observed in plasma and rectal swabs. In thes

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Author

Prezioso, Carla; Obregon, Francisco; Ambroselli, Donatella; Petrolo, Sara; Checconi, Paola; Rodio, Donatella Maria; Coppola, Luigi; Nardi, Angelo; Vito, Corrado de; Sarmati, Loredana; Andreoni, Massimo; Palamara, Anna Teresa; Ciotti, Marco; Pietropaolo, Valeria

Publisher

MDPI AG; Multidisciplinary Digital Publishing Institute (MDPI); Basel: MDPI, 2009- (ISSN 1999-4915)

Published

2020

Language

EN

Field

Medicine (Health Sciences)