About this Earth and Planetary Sciences article
The Energetics Of Extracellular Fe(iii) Reduction By Iron-limited Chlamydomonas Reinhardtii (chlorophyta) by Xiaoping Xue; Christopher M. Collins; Harold G. Weger is a Earth and Planetary Sciences article available to read on EtoBox.
Fe-limited cells of the green alga Chlamydomonas reinhardtii (Fe-limited growth rate ϭ 0.3 d Ϫ1 ) reduced extracellular Fe(III) to Fe(II) when Fe(III) was supplied as ferricyanide or Fe(III)-EDTA; Fe(III) reduction was stimulated by light. In both darkness and during photosynthesis, ferricyanide reduction was accompanied by a decrease in cellular NADPH levels, with a concomitant increase in NADP ϩ . NADH and NAD ϩ levels were not measurably altered during ferricyanide reduction. Furthermore, cellular hexose monophosphate levels declined and 6-phosphogluconate levels increased during ferricyanide reduction. Levels of most glycolytic and tricarboxylic acid cycle intermediates were mostly unaltered. Ferricyanide reduction was also associated with a decrease in cellular ATP levels, a concomitant increase in ADP and AMP, and increased extracellular acidification. The acidification was sensitive to inhibition by the H ϩ -ATPase inhibitor N,NЈ-dicyclohexylcarbodiimide (DCCD). We conclude that the oxidative pentose phosphate pathway provides reducing equivalents for Fe(III) reduction in darkness and also contributes reducing equivalents to Fe(III) reduction during photosynthesis. The decli
It is typically read by researchers, students, and practitioners in Earth and Planetary Sciences.
- Author
- Xiaoping Xue; Christopher M. Collins; Harold G. Weger
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3646)
- Published
- 1998
- Language
- EN
- Field
- Earth and Planetary Sciences (Physical Sciences)