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β -oxidation–polyhydroxyalkanoates synthesis relationship in Pseudomonas putid a KT2440 revisited by Si Liu; Tanja Narancic; Jia-Lynn Tham; Kevin E. O’Connor is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is β -oxidation–polyhydroxyalkanoates synthesis relationship in Pseudomonas putid a KT2440 revisited about?

## Abstract __Pseudomonas putida__ KT2440 is a well-known model organism for the medium-chain-length (mcl) polyhydroxyalkanoate (PHA) accumulation. (__R__)-Specific enoyl-coenzyme A hydratase (PhaJ) was considered to be the main supplier of monomers for PHA synthesis by converting the __β__-oxidation intermediate, trans-2-enoyl-CoA to (__R__)-3-hydroxyacyl-CoA when fatty acids (FA) are used. Three PhaJ homologues, PhaJ1, PhaJ4 and MaoC, are annotated in __P. putida__ KT2440. To investigate the relationship of fatty acids–PHA metabolism and the role of each PhaJ in PHA biosynthesis in __P. putida__ KT2440, a series of __P. putida__ KT2440 knockouts was obtained. PHA content and monomer composition in wild type (WT) and mutants under different growth conditions were analysed. PhaJ4 was the main monomer supplier for PHA synthesis with FA as sole carbon and energy source, with preference towards C8 and C10 substrate, whereas PhaJ1 showed preference for the C6 substrate. However, when all three PhaJ homologues were deleted, the mutant still accumulated PHA up to 10.7% of the cell dry weight (CDW). The deletion of (__R__)-3-hydroxydecanoyl-ACP:CoA transacylase (PhaG), which connects de n

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Author

Si Liu; Tanja Narancic; Jia-Lynn Tham; Kevin E. O’Connor

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)