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Isocitrate Lyase Activity in the Regenerating Forelimb of the Adult Newt by Laura G. Jasch; Anthony J. Schmidt is a Environmental Science article available to read on EtoBox.
What is Isocitrate Lyase Activity in the Regenerating Forelimb of the Adult Newt about?
## Abstract Regenerating and non‐regenerating limb tissues from the adult newt, __Diemictylus viridescens__, were assayed for isocitrate lyase activity. The enzyme assays were performed by micromodifications of existing procedures. In general, the whole homogenate, or a soluble fraction of the homogenate, was incubated with the substrate isocitrate. Isocitrate is cleaved by isocitrate lyase to glyoxylate and succinate. At the termination of the reaction, the 2,4‐dinitrophenylhydrazone derivative of glyoxylate was produced, extracted and quantitated spectrophotometrically. Isocitrate lyase activity was localized to bulb and two‐digit regenerates. The reaction product, glyoxylate (a monocarboxylic keto acid), was of special interest due to its role as a potent in vitro metabolic inhibitor. Therefore, the endogenous level of monocarboxylic keto acids, and the ability of regenerating tissue to produce and accumulate monocarboxylic keto acids, were investigated. Whole homogenates of regenerating tissue always contained more monocarboxylic keto acids than intact forearm or stump tissue. Also, after 30 minutes incubation with buffer alone, only regenerating tissue produced and accumulated
Who reads Isocitrate Lyase Activity in the Regenerating Forelimb of the Adult Newt?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Laura G. Jasch; Anthony J. Schmidt
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)
- Published
- 1974
- Language
- EN
- Field
- Environmental Science (Life Sciences)