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Vicinal Diaminobenzoacridine Used as the Fluorescent Probe for Trace Nitric Oxide Determination by Flow Injection Spectrofluorimetry and Macrophage Cells Imaging by Jixi Hu; Lingling Yin; Kehua Xu; Jingjing Gao; Lili Tong; Bo Tang is a Chemistry article available to read on EtoBox.

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Based on a photoelectron transfer (PET) mechanism, vicinal diaminobenzoacridine (VDABA), a fluorescent probe for the determination of trace amounts of nitric oxide radical in biological sample, was synthesized and characterized by elemental analysis, IR, (1)H NMR and (13)C NMR spectrum. Combining a flow injection with spectrofluorimetry, a high-throughput method for detecting NO was obtained, which was successfully applied to the determination of NO in the human serum. The proposed method was simple, rapid, precise and automatic. Under optimum conditions, the linear calibration range was from 1.1 x 10(-7) to 5.0 x 10(-6)M and the detection limit was 3.1 x 10(-8)M. Furthermore, the probe could make cell-derived NO "visible" by using confocal laser scanning microscope.

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Author

Jixi Hu; Lingling Yin; Kehua Xu; Jingjing Gao; Lili Tong; Bo Tang

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

2008

Language

EN

Field

Chemistry (Physical Sciences)