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Can I read Differential Radiosensitizing Effect of Valproic Acid in Differentiation Versus Self-Renewal Promoting Culture Conditions on EtoBox?
Differential Radiosensitizing Effect of Valproic Acid in Differentiation Versus Self-Renewal Promoting Culture Conditions by Bisrat G. Debeb; Wei Xu; Henry Mok; Li Li; Fredika Robertson; Naoto T. Ueno; Jim Reuben; Anthony Lucci; Massimo Cristofanilli; Wendy A. Woodward is a Medicine article available to read on EtoBox.
What is Differential Radiosensitizing Effect of Valproic Acid in Differentiation Versus Self-Renewal Promoting Culture Conditions about?
Purpose: It has been shown that valproic acid (VA) enhances the proliferation and self-renewal of normal hematopoietic stem cells and that breast cancer stem/progenitor cells can be resistant to radiation. From these data, we hypothesized that VA would fail to radiosensitize breast cancer stem/progenitor cells grown to three-dimensional (3D) mammospheres. Methods and Materials: We used the MCF7 breast cancer cell line grown under stem cell-promoting culture conditions (3D mammosphere) and standard nonstem cell monolayer culture conditions (two-dimensional) to examine the effect of pretreatment with VA on radiation sensitivity in clonogenic survival assays and on the expression of embryonic stem cell transcription factors. Results: 3D-cultured MCF-7 cells expressed higher levels of Oct4, Nanog, and Sox2. The 3D passage enriched selfrenewal and increased radioresistance in the 3D mammosphere formation assays. VA radiosensitized adherent cells but radioprotected 3D cells in single-fraction clonogenic assays. Moreover, fractionated radiation sensitized VA-treated adherent MCF7 cells but did not have a significant effect on VA-treated single cells grown to mammospheres. Conclusion: We h
Who reads Differential Radiosensitizing Effect of Valproic Acid in Differentiation Versus Self-Renewal Promoting Culture Conditions?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Bisrat G. Debeb; Wei Xu; Henry Mok; Li Li; Fredika Robertson; Naoto T. Ueno; Jim Reuben; Anthony Lucci; Massimo Cristofanilli; Wendy A. Woodward
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)