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High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis by Patricia Costaglioli; Eliane Meilhoc; Jean Michel Masson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis about?

A method has been developed for introducing heterologous DNA rapidly and efficiently by electropermeabilization into the yeast Schwanniomyces occidentalis. A transformation efficiency as high as 2 x 10(5) transformants/microgram of plasmid DNA was obtained with a square-wave electric pulse of 2.17 kV/cm during 18 ms. Small quantities of DNA (5 ng) can be used to transform 3 x 10(8) cells. The main parameters which have been optimized are: presence of adenine in the culture medium, pretreatment of the cells with dithiothreitol during the exponential growth phase of the cells, amount of cells treated, and pulse-field strength and duration. Competent cells can be stored to allow electrotransformation whenever needed.

Who reads High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis?

Author

Patricia Costaglioli; Eliane Meilhoc; Jean Michel Masson

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0172-8083)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)